We conducted distance sampling surveys of displaying males across three contrasting landscapes in Sardinia and modelled habitat preference at both the landscape and local (within pastures and recent fallows) scale. Abbasanta, with a balance of pasture and cropland, the greatest isolation from roads and shortest vegetation, supported the highest little bustard densities (95%CI 2.7–3.4 males/100 ha). Significantly lower densities were found in two landscapes with lower isolation from roads and taller vegetation within grasslands: Campeda (0.1–0.2 males/100 ha), comprising cropland and pasture in similar proportions to those found at Abbasanta, and Campidano (0.3–0.4 males/100 ha) that was dominated by cereal agriculture. At the landscape level, males preferred pastures and recent fallows over arable lands. At the local scale, within grasslands, probability of occurrence was greater with shorter vegetation, more legume and green herb cover and at points remote from roads. Shorter vegetation in grasslands resulted from high grazing pressure, and habitat suitability for breeding males depends strongly on extensively grazed grasslands. Conservation efforts for this species should focus on maintaining traditional agro-pastoral practices which maintain large areas of extensively grazed pastures and recent fallows located far from roads.